Examinando por Autor "López-Rojas, L. E. (Luis Ernesto)"
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Publicación Acceso abierto La Biotecnología y el desarrollo de investigación aplicada en el programa de Ingeniería Biomédica de la EIA y el CES(2014-04-20) López-Rojas, L. E. (Luis Ernesto); García-Quiroz, F. (Felipe); Londoño-Peláez, C. (Carolina)Publicación Acceso abierto Cultivo de tejido cartilaginoso articular: acercamiento conceptual(2014-05-08) Zapata-Linares, N. M. (Natalia María); Zuluaga, N. J. (Natalia Janet); Betancur-Gallego, S. N. (Silvia Natalia); López-Rojas, L. E. (Luis Ernesto)The currently available methods for tissue repair have not been able to restore completely functional cartilage tissue. For this reason, tissue engineering has developed strategies for fabricating cartilage substitutes in order to offer therapeutic solutions to patients that could suffer from any kind of cartilage disease. The purpose of this article was to review the anatomy, histology, physiology, pathology of cartilage, and the therapies commonly used for repairing this tissue. This article also shows the role established by tissue engineering and biomaterials in this field.Publicación Acceso abierto Ingeniería de tejido óseo: consideraciones básicas(2014-05-07) Estrada, C. (Catalina); Paz, A. C. (Ana Cristina); López-Rojas, L. E. (Luis Ernesto)Usually bone tissue loss caused by trauma, osteonecrosis, and tumors has been treated using autograft, allograft, xenograft, and implantation of substitute materials. Each of these treatments has significant limitations, like availability of sufficient donor tissue, disease transmission, donor site morbidity, and inability of materials to remodel and react against physiological conditions. For all these reasons there is a need for alternative bone replacement procedures. Tissue Engineering aims to satisfy this need by the development of bone substitutes using different cell types, three-dimensional matrixes (scaffolds), in a medium supplemented with growth factors. The present review is focused in Bone Tissue Engineering and its most important aspects.Publicación Acceso abierto Isolation of human bone marrow mesenchymal stem cells and evaluation of their osteogenic potential(2014-04-23) García-Quiroz, F. (Felipe); Posada-Estefan, O. (Olga); Gallego Pérez, D. (Daniel); Higuita-Castro, N. (Natalia); Sarassa-Velásquez, C. (Carlos); Hansford, D. (Derek); Agudelo-Florez, P. (Piedad); López-Rojas, L. E. (Luis Ernesto)Human bone marrow mesenchymal stem cells (hBMSCs) comprise a cell population capable of self-renewal and multilineage differentiation commonly isolated from bone marrow aspirates of large bones. Their osteogenic potential has been extensively exploited for the biological evaluation of scaffolds or biomaterials with applications in bone tissue engineering. This work aimed to isolate hBMSCs from femoral heads of patients undergoing total hip arthroplasty and to evaluate their osteogenic potential. Briefly, the trabecular bone was extracted and mechanically disaggregated; the released cells were cultured and non-adherent cells were removed after 4 days. The osteogenic potential was evaluated at the fifth passage after 14 and 20 days of induction, comparing cultures with and without osteogenic supplements, via Alizarin red staining and the quantification of the gene expression levels of the osteogenic markers collagen type I, osteonectin and bone sialoprotein through real-time RT-PCR. The obtained hBMSCs presented a stable undifferentiated phenotype after prolonged cell culture, matrix mineralization capabilities and expression of osteoblast phenotype upon osteogenic induction. The three markers were up-regulated in cultures under osteogenic conditions and 2 fold differences in their expression levels were found to be significant for the onset of the differentiation process. The obtained hBMSCs may have applications on the in vitro evaluation of the osteoinductivity of different biomaterials, bioactive molecules or tissue engineering scaffolds.